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JAMA (1991) 265(1):945.10.1001/jama.265.1.94 8 LederleFA

After centrifuging at 4000 g for 20 min, sediment was resuspended in phosphate-buffered saline (PBS, 1/10 of original volume) and stored at -80 C

Therefore, the lack of significant TNF- changes observed in this study might be due to both the methodological limitations of IHC and moderate inflammation severity during the 21-day induction period