Research Article Other versions: (Verbatim name) ID (Catalog Number) Invalid DOI was provided, please try again ContentsContents Article InfoArticle Info CiteCite MetricsMetrics CommentComment RelatedRelated FigsFigs TabsTabs MapMap TaxaTaxa DataData RefsRefs CitedCited Article title Abstract Key words Introduction Materials and methods Fungal strains Culture conditions Breeding programme aimed to develop divergent fast- and slow-growing bred lines Construction of bidirectional hybrid strains Nuclear and mitochondrial DNA extraction and characterisation of molecular markers Analysis of quantitative and molecular traits in parental and hybrid strains Growth rate of parental and hybrid strains under different temperatures and carbon sources Analysis of mushroom production in hybrid strains Qualitative determination of antioxidant compounds in fruiting bodies ROS detection in parental and hybrid strains RNA extraction, RT-qPCR conditions and analysis of gene expression Sequences of genes analysed in this work Modes of inheritance and heterosis levels Statistical analysis Declaration of AI-assisted technologies in the writing process Results Molecular markers for the identification of nuclear and mitochondrial genomes A divergent selection programme aimed at maximising phenotypic variation in growth rate Construction of hybrid strains Analysis of growth rate under standard conditions (SC, 24 C) and at 15 C and 32 C in parental and hybrid strains Analysis of growth rate on different carbon sources in parental and hybrid strains Expression analysis of the ETC and detoxification-related genes in parental and hybrid strains Expression analysis of genes belonging to different complexes of the ETC Complex I nd1 expression Complex III bcs1 and rip1 expression Complex IV cox4 and cox5b expression Analysis of the expression profiles of detoxification-related genes Determination of oxidative stress by analysing ROS using probes detecting H2O2/peroxides in parental and hybrid strains Analysis of the influence of the mitochondrial genome on quantitative traits related to mushroom production in hybrid strains Discussion Conclusions Additional information Conflict of interest Ethical statement Use of AI Adherence to national and international regulations Funding Author contributions Author ORCIDs Data availability References Supplementary material Subscribe to email alerts for current Article's categories
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