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Indeed, at least 80% of GABA secretion from chick retinal cell occurs through GAT-1/3-like transporters.Citation 31 Also, data from our group show that GAT-3 transporter is regulated by glutamate in Mller glia.Citation 33 In summary, ATP induces Ca 2+ increase that might lead to neurotoxicity in retinal neuronal-glial cell cultures,Citation 34 but GABA released by GSH through a P2X7 glial receptor could represent a mechanism of protection in the avian retina, where Mller cells have a pivotal protective role

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Care must be taken to ensure the needle does not penetrate too deeply to prevent potential organ damage