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15 g of lysates were reduced in NuPAGE reducing reagent (Invitrogen) and boiled for 5 min prior to loading in 4-12% NuPAGE Bis-Tris Gel for separation by electrophoresis

(Source 2, 3) "The primary downside of iron supplementation is the high rate of potential side effects, particularly gastrointestinal side effects, including nausea, vomiting, and constipation." Potential Side Effects of Iron Iron supplements, especially when taken without food on an empty stomach, can sometimes cause side effects such as constipation, nausea, stomach cramps, or a metallic taste-especially with traditional forms like ferrous sulfate