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Attenuating ischemia-reperfusion injury in kidney transplantation by perfusing donor organs with siRNA cocktail solution

In particular, MPTP intoxication typically downregulates key autophagy regulators (LC3-II, beclin-1, parkin, PINK1), but schisandrin A treatment partially preserved the levels of these proteins, implying that it enhances autophagic clearance of toxic aggregates (59, 60)

Determination of leaf malondialdehyde and H 2 O 2 2-thiobarbituric acid (TBA) was used to determine malondialdehyde (MDA) following the method of Hodges et al

GGT activity measurement GGT activity was measured employing the GGT activity colorimetric assay kit provided by Sigma-Aldrich (MAK089) following manufacturers instructions

Model robustness was assessed using Receiver Operating Characteristic - Area Under Curve (ROC-AUC) analysis in MetaboAnalyst software

Finally, Novotny et al