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Methods Reagents and tools table Maintenance of human ESC lines HES3 NKX2-5 eGFP/w hESCs (Elliott et al, 2011) and gene-edited derivatives were maintained on a layer of mitotically-inactivated mouse embryonic fibroblasts (MEFs) in DMEM/F12-based medium containing 20% (v/v) KnockOut Serum Replacement (Gibco), 100 mM non-essential amino acids (Gibco), 2 mM GlutaMAX (Gibco), 0.1 mM -mercaptoethanol (Gibco) and 10 ng/ml bFGF (Miltenyi Biotech)

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This binding can trigger intracellular signaling that: Enhance epithelial cell proliferation and migration, promoting mucosal healing

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Some studies have shown that saikosaponin D can increase the survival rate of HK-2 cells and maintain the normal morphology of the nucleus

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