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First, we sought to identify a medium that would support assembloid formation, that is, the culture of all three cell types: hepatocytes, cholangiocytes/ductal cells and portal mesenchyme without overgrowth of any of them, we tested several media and found that a minor adaptation of the DM medium used for h-HepOrgs differentiation without A8301 (assembloid medium) supported culture of the three cell types while preventing their overgrowth

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Thymosin beta-4 binds to monomeric G-actin with a 1:1 stoichiometry, forming a complex that prevents spontaneous polymerization of actin into filaments (F-actin)

& Ishizaka, K