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Cells were resuspended in IBB Lysis Buffer (20 mM Tris-HCl, pH 8.0, 200 mM NaCl, 1 mM PMSF, 3 mM 2-mercaptoethanol, 1 mM EDTA, and 0.1% Triton X-100, DNase, and RNase) and after sonication, the soluble fraction was bound to amylose resin (New England Biolabs)

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Collectively, the selenoprotein translation machinery consists of SECIS element, SBP2, Sec-specific eukaryotic elongation factor (EEFSEC), and aminoacylated Sec-tRNA [Ser]Sec , thereby enabling UGA to be recognized as a Sec codon and utilized for translation into the growing polypeptide

Denpasar: Universitas Udayana;2014

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