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During pregnancy, the potential "perks" of a well-supported antioxidant system include: Support for Cellular Health: By neutralizing free radicals, glutathione helps maintain the integrity of cells in both the mother and the developing baby

RNA quantity and quality were assessed via standard spectrophotometric methods and Agilent 2100 Bioanalyzer (Santa Clara, CA

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Methylene Blue acts as a robust antioxidant, helping neutralize free radicals and protect the complexion from oxidative stress

Total RNA was extracted using the AccuPrep Universal RNA Extraction Kit (Bioneer, Seoul, South Korea)

Relative level of ROS was detected centrifuging liver tissue in the appropriate buffer (20 mmol/L Tir-HCl (pH 7.4), 20 mmol/L NaH 2 PO 4 , 5 mmol/L MgCl 2 , 130 mmol/L KCl and 30 mmol/L glucose) and incubating the supernatant with DCFH-DA (2, 7-dichlorodihydrofluorescein diacetate) for 15 min at 37 C