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Although no significant adverse effects were observed in our study, including hemolysis, histopathological changes, and Mn ion accumulation, the potential risk of LAbs inducing tumorigenicity or undesired immunological responses following repeated administration should be systematically investigated

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Ascorbic Acid is Vitamin C which is another antioxidant

The precipitated plasmid was collected by centrifugation at 16,000 g for 10 min at room temperature, followed by washing with 800 L of 75% (v/v) ethanol (in di-distilled water) twice

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