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1f and Fig S1d)

Cells were resuspended in the same medium, rested at 37 C for 0.51 h, filtered through 100-m strainers (VWR) and dispensed at 1 10 5 neutrophils per well (500 l) in 24-well ultra-low attachment plates

this enhanced heat resistance was related to the expression of starvation-induced proteins UspA and GrpE (Zhang and Griffiths, 2003)

Glutathione is present in high concentrations in cultured astrocytes but not in cultured neurons

Experimental validation Dose response curves and determination of IC 50 values were assessed for the 12 candidate drugs using 3-fold dilution series (see Supplementary Methods (Supplementary File 1) for more details)