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Similarly, in weaver mice where the spontaneous depletion of dopaminergic neurons occurs, nNOS is increased in the substantia nigra but not in other brain regions 13

Jama, 262(11), 1479-1484

To compensate for the resulting lack of cellular energy, podocytes enhance their amino acid metabolism to increase intermediates of the TCA cycle and maintain OXPHOS

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Proximity ligation assay Duolink in situ Proximity Ligation Assay (Sigma Aldrich, USA) permits the detection of proteinprotein interactions

Combining the tests provided a diagnostic sensitivity of 100%, a specificity of 65.5%, and an AUC of 0.909 (95% CI: 0.8310.988) for diagnosing WD cirrhosis (Fig