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Nitric oxide synthase 2 (NOS2) is another major catabolic enzyme for L-Arg metabolism in MDSCs (105)

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This phenotype could be restored in the complementation strain C gsh-px

Future studies assessing Keap1 expression, incorporating human samples and using Nrf2-deficient models, together with measurements of apoptosis and autophagy markers, will help strengthen the mechanistic interpretation

Electroporation buffer and unloaded RFP were removed by ultrafiltration via an Amicon centrifugal filter (Millipore, UFC510096, 100 kDa molecular weight cutoff) followed by three washes with DPBS buffer (10 mM, pH 7.4, 13,000 g) at 4 C