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The macromolecular receptor was processed using AutoDockTools 1.5.7 software, including the addition of hydrogen bonds, calculation of charges, setting of rigid structures, and designation as the receptor, and then exported in PDBQT format

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[Drug Metabolism and Disposition, 2013, vol

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The resulting PV2-S71 + PV3-N77 bispecific nanobody (BsNb-ncAA) exhibited higher thermostability and binding affinity compared to the nanobody monomers and the BsNb constructed by simply fusing two proteins