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The promoter region of OLFM4 was amplified with the primers: GCTCA GGTACC TTCCTCCATGGAGCCTCCAAAC (Kpn1 site) and GCTCC AAGCTT CCACTCTGTGAGCTGCCCTTGG (HindIII site), and was subcloned into the pGL3 basic firefly luciferase reporter vector

Van Soest PV, Robertson J, Lewis B

Liang G, Lin JCY, Wei V, Yoo C, Cheng JC, Nguyen CT, et al

Xia Q, Chen Y, Yu Z, Huang Z, Yang Y, Mao A

However, patients with pre-existing liver disease should be monitored appropriately, and any unexplained symptoms such as jaundice, dark urine, or persistent right upper quadrant pain warrant prompt medical evaluation

In addition, the ROS produced by ATMs first affect their recruitment and polarization, especially in cases such as diabetes, ROS induces ATMs to polarize towards M1 and the release of inflammatory factors (145, 146)