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The cells were resuspended in relaxation buffer (100 mM KCl, 3 mM NaCl, 3.5 mM MgCl 2 , 1 mM ATP and 10 mM PIPES pH 7.3) containing protease inhibitors (cOmplete EDTA-free, Roche)

Autophagy (2021) 17:205481

Most patients barely notice them

Additionally, estrogens seem to be necessary for axonal growth and synaptic plasticity (Kretz et al., 2004

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Stimulation of steroidogenesis in immature rat Leydig cells evoked by interleukin-1alpha is potentiated by growth hormone and insulin-like growth factors