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Immunohistochemistry Anesthesia was induced with 5% isoflurane in an induction box and maintained with 1.5% isoflurane via a face mask throughout the operation

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Under basal or lipotoxic conditions, G9a represses autophagy by enriching H3K9me2 at the promoters of LC3B, WIPI1 , and DOR , thereby silencing their transcription and reducing their autophagic activity

Singh et al., 2009) which can induce lipid peroxidation with the production of toxic products (Inamdar et al., 2010)

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