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Springer International Publishing, Cham, pp 195211

14 PCR amplification was performed in a final volume of 25 l of the reaction mixture containing 1.5 U of Taq DNA polymerase (Sibenzyme), about 1 g DNA of each patient's DNA, 0.25 M each primer, 250 M of dNTPs, 2 mM of MgCl 2 , 1 PCR buffer of the following composition: 67 mM Tris-HCl pH 8.8, 16.6 mM (NH 4 ) 2 SO 4 and 0.01% Tween-20

This finding supports the hypothesis that hyaluronic acid injections can not only improve symptoms but also protect cartilage and prevent further cartilage degradation (27)

Further dose increases: After at least 4 weeks on the current dose, the dosage may be increased in 2.5 mg increments

On average, effects can last anywhere from a few weeks to a few months