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Moreover, OPLS-DA models were validated by internal iterative cross-validation with seven rounds of permutation test response (800 repeats) not clear, and CV-ANOVA (ANOVA testing of cross-validated predictive residuals)

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Prepared solutions are typically maintained under refrigerated conditions at 36-46F (2-8C) or as specified by the research protocol, with aliquoting commonly used to help preserve sample integrity during storage