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For the knockdown of genes that cause embryonic lethality, RNAi was started at L4-stage animals, and the animals were allowed to develop further for 48 h at 20 C or otherwise indicated

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Metformin attenuates diabetes-induced tau hyperphosphorylation in vitro and in vivo by enhancing autophagic clearance

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This marks an important step forward in stroke research and potential treatment development (Liu S

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Subsequently, single-stranded cDNA was synthesized from the RNA using reverse transcriptase M-MLV (D2640A, TaKaRa, Dalian, China)

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