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The insoluble portion was subsequently removed by centrifugation and the supernatant was incubated with Ni-NTA resin (GE Healthcare) for 2 h batch binding at 4 C

Excess water can dilute the serum, reducing its effectiveness

Genetic Factors Alteration of the Transsulfuration Pathway One of the classic alterations of the homocysteine metabolism is represented by the homozygous deficiency of CBS (Mudd et al, 1995)