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Primary and secondary antibodies were diluted in blocking solution (0.3% (v/v) Triton X-100, 10% (v/v) FBS in PBS)

Once the rats were killed, the colon was removed aseptically and placed on an ice-cold plate, longitudinally opened and the luminal contents were collected for the measurements of faecal moisture, pH and microbiological and SCFA production studies (see later)

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Healthcare providers most commonly recommend IV therapy during pregnancy for specific medical situations where the benefits outweigh any potential risks

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