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Cancer Cell 2013;23:739-52

Tissue sections were incubated with primary mouse antibodies FITC anti-CD192 (1:100 dilution) (CCR2) (BioLegend, 150607), PE anti-CD11b (1:200 dilution) (BioLegend, 101208), anti-CD68 (1:200 dilution) (BioLegend, 137001), anti-F4/80 (1:200 dilution) (BioLegend, 123101), and anti-IL-6 (D5W4V) (1:200 dilution) (Cell Signaling 12912S) overnight, stained with secondary antibody (for CD68, F4/80, IL-6) (Invitrogen SA5-10018, A21434 and A11036)

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wherein A68 is substituted with either a G, I, L, M, S, T, or V

J., Choi, K