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This was performed by treating cells with or without an mTORC1 activator (insulin, 15 minutes treatment), and by additionally treating with or without an mTORC1 inhibitor (rapamycin) for three hours in insulin-deprived cells (repeated three times)

Tell your clinician if you have active cancer, a history of malignancy, uncontrolled diabetes, severe sleep apnea, or pituitary disease, as GH/IGF-1related therapies may be inappropriate in some cases