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Golden Gate reaction mixtures were prepared as follows: 40 fmol of each DNA insert or plasmid, 1.5 l of T4 DNA ligase (400 U/l, NEB), 1 l of restriction enzyme, 2.5 l of 10 ligation buffer, and sterile water to bring the final volume to 25 l

The solution is to determine your actual concentration (10mg divided by actual mL added) and use the unit conversion formula (dose in mg / concentration in mg per mL x 100) to recalculate your correct draw volume

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The anti-apoptotic activity of TB-500 may involve the reduction of early cell death initiation signals and activation of the survival kinase Akt through complex formation with PINCH and integrin-linked kinase

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