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Moreover, APP-CTF is a suspected factor mediating the increased late-onset AD risk due to polymorphisms of the neuronal lysosomal protein phospholipase D3 (PLD3): PLD3, a transmembrane protein delivered from the Golgi and proteolytically cleaved in lysosomes, yields a stable soluble polypeptide with 53 exonuclease activity but unclear roles in lysosomes 142

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X., Xu, J., Zhao, E

Dimethyl sulfoxide diluted in a .01 M glycine buffer was added to solubilize the cell membranes and the absorbance of reduced MTT in the buffer was quantitated at 590 nm using a plate reader (Biotek Synergy 2, Winooski, VT)

As an NADPH-dependent oxidoreductase of CoQ10, the N-terminal myristoylation of FSP1 can promote its targeting of the plasma membrane and mediate NAD(P)H-dependent CoQ10 reduction, thereby inhibiting the activity of CoQ10

Zero-valent iron nanoparticles (ZVI-NP) can target and enhance the degradation of nuclear factor-E2-related factor 2 (NRF2), inducing ferroptosis in lung cancer cells through the Fenton reaction (with MDA levels increasing exponentially), while polarizing M2-type tumor-associated macrophages (TAMs) to M1-type and reducing the infiltration of regulatory T cells (Tregs) by half