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10d), but appeared fully dependent on it for the addition of scTS2/16

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10.1111/cpr.13134 165 ZhouY.QueK.-T.ZhangZ.YiZ

Quantitative real-time PCR Total RNA was prepared using Tri-RNA reagent (FAVORGEN Biotech Corp., Taiwan) and complementary DNA (cDNA) was synthesized using the PrimeScript RT Reagent Kit (Takara Bio Inc., Japan) according to the manufacturers instructions