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Furthermore, different studies employ varied sequencing methods (e.g., ITS1, ITS2, or 18S rRNA) and rely on fungal reference databases (e.g., UNITE, ITSoneDB) that are continuously updated 107,108 , which may introduce biases in species classification, lead to underdetection of low-abundance taxa, and complicate direct comparisons across studies

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Further, plasma levels of d3-BB were significantly higher than that of either d3-TMA or d3-TMAO, again indicating formation of BB was a major metabolite formed from dietary L -carnitine

Current literature suggests that the L-carnitine system is perturbed in AD, with multiple studies showing that proteins and metabolites related to the L-carnitine system are altered in the brain, CSF, and plasma of AD patients (Makar et al., 1995

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