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From Our Lecture Notes: Neuroinflammation is characterized by multiple interwoven features , including microglial activation, increased glial polarization (M1 subtype), blood-brain barrier permeability, and infiltration of peripheral immune cells

the slices were deparaffinized and stained with hematoxylin a After deparaffinization, the slices were stained with hematoxylin and eosin (H.E.), and the slices were sealed and observed and photographed with an orthogonal fluorescence microscope (Leica DM5500B)

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100 Consistent with this, paracellular flux of [ 3 H]mannitol (hydrodynamic radius of 3.6 ), but not [ 14 C]inulin (hydrodynamic radius of 11.5 ), was also increased, indicating that a decrease in TER resulted from tight junction-dependent permeability, not epithelial damage

Second-messenger and pathway readouts: Experimental models may assess pathway outputs (assay-dependent) to profile signaling behavior and compare conditions