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While proper loading time is relatively easily to establish and loading temperature is mostly the temperature used for cell culture (for example 37 C and 39 C for human and mouse cells and cell lines, respectively), additional fine-tuning is needed to establish proper loading concentration of the fluorescent dyes to avoid mitochondrial toxicity, non-specific binding to other cellular structures, photodamage and dye aggregate artifacts

This leads to an overabundance of calcium ion (Ca 2 ) influx and subsequent excitotoxic neuronal injury, contributing to ototoxicity

doi: 10.1016/j.redox.2023.102643 76 HenrotinYKurzBAignerT

Specifically, glutathione: Fights off toxins such as mercury in the liver and brain Regenerates vitamin C and E in the body for strong immune support Is essential for energy production and cell detoxification Improves protein and enzyme levels in the blood Protects and supports cellular function, especially in the liver (part of the immune system) Oxidative stress biomarkers such as glutathione can test for a variety of diseases (2)

187 Green tea contains valuable antioxidant polyphenols known to be protective against a host of chronic age-related conditions

Funding The present study was supported by the Tianjin Health Commission, Research Project of TCM and Western Integrative Medicine (grant no