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Both animal and human studies have shown no toxicity or adverse effects even at high ergothioneine doses

and is highly associated with poor long-term patient outcomes, including death and the need for RRT 30 days after surgery [32, 33]

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The FDR was calculated using a subset of false-positive proteins expected not to be labeled by LaccID: nucleus and cytosol proteins that werenot annotated with terms related to the cell surface, Golgi or membrane (Supplementary Table 4)

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ReceptorLigand Binding Assays The peptide serves as a selective ligand in receptor binding studies to evaluate GHSR-1a affinity, receptor density, and activation thresholds under controlled assay conditions