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Testing also showed that the measure far exceeded the 0.4 threshold for mean reliability, which we reaffirmed as the threshold for reliability in the CY 2022 PFS final rule (86 FR 64996)

[DOI] [PubMed] [Google Scholar] 73.Martinsen T.C., Fossmark R., Waldum H.L

47 NencioniL.IuvaraA.AquilanoK.CirioloM

[DOI] [PMC free article] [PubMed] [Google Scholar] 180.Gonalves CL, Doifode T, Rezende VL, et al

Next, after 3 washings with PBS-Tween 0.1% and 2 washings with the B/W buffer for 5 minutes at RT, 8 pmol of ssDNA pool from the rounds of selection were added to each dot and incubated for 1 hour at 22 C, then washed 3 times with the B/W buffer