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doi: 10.1038/s41586-021-03707-9 79 HanH.WolffE

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For all in vitro assays the NAPstar sensor protein was quantified by Bradford assay [68] and the sensor protein was used at a final concentration 240480 nM

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Early-life HFHS diet-exposed adult males displayed alterations in microbiota-derived metabolites including the secondary bile acid deoxycholic acid, and the tryptophan metabolite formylkynurenine, as well as in the host-derived metabolites tryptophan, serotonin, thiamine, succinic acid, and the primary bile acids cholic acid and -muricholic acid (Fig