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Sulforaphane activates Phase II detoxification genes, enabling the conversion of stored flame-retardants, combustion hydrocarbons, and plasticizers into water-soluble forms ready for elimination, while also lowering inflammatory stress on detox organs

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The enzymatic reaction was initiated by adding 1 mL of GPx enzyme solution (3000 units/liter) to 9 mL of a mixture containing potential interfering substances

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The mixture was incubated on ice for 15 min and briefly vortexed at 5-minute intervals during incubation to enhance membrane disruption and lysis efficiency