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Recombinant proteins were expressed in Escherichia coli BL21-CodonPlus (DE3) cultured in LB medium, after induction with 0.2 mM IPTG overnight at 16 C, the bacteria were pelleted by centrifugation at 4000 g for 10 min

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While inhibition of NUPR1 by the specific inhibitor ZZW-115 dose-dependently decreased epithelial barrier integrity and induced cytotoxicity, none of these readouts were modulated by CS exposure in vitro