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The above experiments show that PC has a widely suppressive effect on various tumor cells, which has crucial clinical significance

Methods The optimized pranlukast-containing amorphous solid dispersion, developed using design of experiments (DOEs), was evaluated for its pharmaceutical and biopharmaceutical properties through various analytical methods, including hot-stage microscopy, thermogravimetric analysis, differential scanning calorimetry, solubility measurement in polymer-containing aqueous solutions, Fourier transform infrared spectroscopy, powder X-ray diffraction (PXRD), dissolution testing, and in vivo pharmacokinetic (PK) studies

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In addition, therapeutic timing and long-term outcomes remain underexplored

L-methylfolate is the natural, non-toxic active form of folate and methylcobalamin is the natural, non-toxic methylated active form of cobalamin

After randomization, participants completed monthly mid-study visits at 1-month (Month 1), 2-month (Month 2), and the end of study visit at 3 months (Month 3) for the final measurement of outcomes of interest, including the final MR scan