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Furthermore, the relative organ weights (g/g %) for all these organs were also recorded by the following equation 41

RBD was purified from the cell culture supernatant using Ni-NTA agarose beads (Qiagen, USA)

The overexpression of NAMPT cDNA in glioma or colorectal cells increased their tumorigenic properties, as well as their acquisition of the CSC phenotype and properties in culture