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Global burden of sickle cell anaemia in children under five, 2010-2050: modelling based on demographics, excess mortality, and interventions

Culture media and conditions Lysogeny broth (LB) medium containing yeast extract (5 g L 1 ), tryptone (10 g L 1 ), and NaCl (10 g L 1 ) was used for inoculant preparation and cell propagation