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found a novel FBXL4 frameshift mutation (c.993dupA) in a Han Chinese patient, leading to the premature termination of protein synthesis and defects at the protein level (110)

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Cells were grown in complete medium to an optical density of 0.4, then washed twice in phosphate buffer (PBS), centrifuged, the supernatant was removed, and resuspended in 100 l of PBS

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