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In another set of experiments, animals (n = 15 for each group) were randomly divided as described above and monitored for assessing mortality for 5 days after a lethal dose of APAP (600 mg/kg) or saline administration

Unlike N -linked glycosylation, for which glycosylation occurs in a specific consensus sequence (Asn-X-Ser/Thr, where X is any amino acid except Pro), no definitive consensus sequence has been identified for O -GlcNAc

The lysates (2 g of protein per lane) in RIPA buffer (Sigma-Aldrich, Cat# R0278) containing a protease inhibitor cocktail as well as phosphatase inhibitor cocktails 2 and 3 (Sigma-Aldrich) were subjected to SDS-PAGE on 520% gradient gels (SuperSep Ace, FUJIFILM-Wako) followed by immunoblotting

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Despite its recognized position in the treatment of neurodegenerative diseases, there is still controversy over the exact mechanism of its action, as well as its clinical effectiveness and application