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( d-e ) Cells were transduced with shGFP (shCont) or shRNAs targeting TMLHE ( d ) or treated with mildronate (Mildro

The first feedlot (control group, CTRL) received a completed pelleted diet (CPD) ad libitum and the second feedlot (carnitine group, CARN) was fed the same ration formulated with 6 g of Carniking (50% L-Carnitine, 35% silica, and 15% water, Lonza) per kg

The homogenates were then spun at 13,200 rpm at 4 C for 30 min to remove debris

This method belongs to a category of weight loss approaches that do not require dieting, do not cause anorexia, fatigue, or diarrhea