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Moreover, it was shown experimentally that Gch1 (gene encoding the rate-limiting enzyme of BH4 biosynthesis) is a direct target of Nrf-2 in skin cells submitted to radiation (Nrf-2 has a binding site in the proximal promoter of the Gch1 gene), and the resultant increased intracellular BH4 levels promoted cytoprotection by neutralizing ROS [108]

The cells were washed twice in 1 mL ice-cold PBS and lysed with 5 pellet volume of RIPA buffer (20 mM HEPES, 100 mM NaCl, 0.1% Triton-X, 0.2% SDS, PhosStop, cOmplete inhibitor tablet)

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Am J Hematol 95:338342

135 The increased basal activation levels of aged DCs disrupt respiratory epithelial function by altering cytokine and chemokine secretion, contributing to chronic airway inflammation and heightened susceptibility to respiratory infections in elderly individuals

These functions highlight its significance in maintaining optimal health and longevity