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Eluted RNA was quantified using a Nanodrop 2000 spectrophotometer, and each RNA sample was diluted to the lowest yield before RT-PCR

This is due to several factors that differ between the controlled conditions of in vitro studies and the complex biological processes in living organisms, including interactions between different cell types, organ systems, and environmental variables (e.g., gut microbiota or circulation) that can influence how a drug works

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The role of ROS in sperm DNA damaging has further been supported by the significant and positive correlations observed between the levels seminal ROS and the proportions of spermatozoa with fragmented DNA (95, 96)

*Correspondence: Xiao-Ping Wang, [email protected] This article was submitted to Molecular and Cellular Pathology, a section of the journal Frontiers in Cell and Developmental Biology Disclaimer All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers