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Streptavidin is tetrameric and can bind up to four biotin molecules, making it useful for surface immobilization, bead capture, and signal amplification

Thus, the ATP depletion and mitochondrial ROS burst observed upon SLC25A39 knockdown are expected to blunt PI3K/AKT signaling and subsequently inhibit mTORC1, driving the observed cell death

SKT, RS and JK provided technical suggestions and reviewed the manuscript

R747W is a mutant phenotype with loss-of-function of the gene encoding iPLA2, which reduces the hydrolytic activity of 15-HpETE-PE, and increases the content of 15-HpETE-PE in cells, and increases the sensitivity to ferroptosis compared with the wild type

Spectra data were log-transformed and weighted by an adaptive intensity weighting algorithm

Methods 8 , 659661 (2011)