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Mol Cell Endocrinol 248 , 182191 (2006)

#13 Use a downward angle maybe even a 45 degree angle at the site and you should be able to keep it subq

C-met binding was examined at two HGF-doses (250 ng/ml and 500 ng/ml in PBS) by sandwich ELISA-like assay on recombinant c-met-Fc chimera as in Figure 1e, and significant changes in optical absorbance subtracted by a baseline level (negative control without HGF, bar 1 ) were depicted in Figure 2a (left column)

Minced tissue was then incubated in 15-ml conical tubes containing Hanks Balanced Salt Solution (HBSS

reverse, 5-TGCCTTTGTTCTTGGCAGAA-3, CCL21: forward, 5-GCAAAGAGGGAGCTAGAAAACAGA-3

Natl J Physiol Pharm Pharmacol