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Cells were incubated in OptiMEM medium supplemented with 5% FBS and 30 ng/ml M-CSF post-infection, and fresh medium was added every 48 h

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ALP mutations Subnormal ALP levels have been reported in patients with hypophosphatasia (HPP), a rare inherited systemic metabolic disease caused by mutations of the tissue-nonspecific ALP ( TNSALP ) gene