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Hematopoietic ABCA1 deletion promotes monocytosis and worsens diet-induced insulin resistance in mice J Lipid Res 57 1: 100-8

Following this, we washed the samples twice with PBS for 10 minutes each time and then stained them with NucBlue (Thermo Fisher Scientific, Waltham, MA, USA) for 3 minutes, followed by a 5-minute wash with PBS