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sample tube) and 200 L of the supernatant was added to a tube with 800 L 2.5 M HCl (control tube), both tubes were incubated in the dark for 1 h with occasional vortexing

Among these are several proteins with key roles in preventing T2D, including carboxypeptidase D (CPD) with potential role in proinsulin processing (166, 167), arsenite-stimulated ATPase (ASNA1) which participates in insulin secretion (168, 169), vascular endothelial growth factor receptor-2 (VEGFR-2) whose soluble form is elevated in plasma of T2D and is associated with insulin resistance (170), mitogen-activated protein kinase kinase kinase 4 (MAP3K4), MAP3K5 (ASK1), and sodium/potassium-transporting ATPase subunit alpha-1 (ATP1A1), each of which harbors SNPs associated with T2D (171173)

doi: 10.1016/j.jsbmb.2017.02.007 76 MellorKMVarmaUStapletonDIDelbridgeLM

Evidence for iNOS-dependent peroxynitrite production in diabetic platelets

Plis SM, et al

When adding water to the lyophilized powder, aim the stream along the inside wall of the vial rather than directly onto the powder