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Since both accumulation and complete elimination of BACE1/A adversely affect recovery after SCI, biological regulation of A or BACE1 levels after SCI by modulation of components of the UPS such as Fbx2-Uch-L1 via peroxisome proliferator-activated receptor-gamma co-activator (PGC)-1alpha or gene expression (Gong et al., 2010, 2013b) may be a feasible strategy for the SCI treatment and AD (Figure 3)

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Briefly, meninges were incubated for 30 min at 37 C in RPMI 1640 media (Gibco) supplemented with 5% FBS (Biological Industries), collagenase IV (1.2 mg/mL, Sigma-Aldrich), DNAse (75 g/mL, Roche), and HEPES (6 mM, Biological Industries)

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