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To set up binding reactions, the mixtures of 20 nM FAMBRD BD1 and 100 nM YPEL5WDR26 CTLH or WDR26 CTLH in the binding buffer (25 mM HEPES pH 7.5, 150 NaCl, 5 mM DTT, 1 mg ml 1 BSA and 0.1% Tween-20) were combined with equal volumes of a twofold dilution series of the degraders or JQ1 (prepared in DMSO and then diluted with the binding buffer to reach the final DMSO concentration of 2% for all titration points) and incubated for 1 h at room temperature before transferring to 384-well flat-bottom black plates (Greiner)

10.1016/j.cca.2009.03.002 57

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The Signaling Model (BPC-157) : This model focuses on the catalytic nature of the drug

Jun HT, Sun J, Rex K, Radinsky R, Kendall R, Coxon A et al